Journal: Diagnostics

Article Title: Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA

doi: 10.3390/diagnostics13030462

Figure Lengend Snippet: ROC curve of the EDIII IgG ELISA showing the evolution of sensitivity and false positive rate at each OD value.

Article Snippet: For indirect IgG ELISA, standard ELISA plates (Immulon II 96-well microtiter plates; Dynatech laboratories, Inc., El Paso, TX, USA) were coated with 100 μL of inhouse-prepared mouse hyper immune ascitic fluids specific to either YFV, DENV, WNV, or ZIKV at 1/1000 dilution in phosphate buffer saline (PBS) solution at 0.135 M and coated overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Diagnostics

Article Title: Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA

doi: 10.3390/diagnostics13030462

Figure Lengend Snippet: Advantages and drawbacks of EDIII ELISA in comparison to standard IgG ELISA and PRNT.

Article Snippet: For indirect IgG ELISA, standard ELISA plates (Immulon II 96-well microtiter plates; Dynatech laboratories, Inc., El Paso, TX, USA) were coated with 100 μL of inhouse-prepared mouse hyper immune ascitic fluids specific to either YFV, DENV, WNV, or ZIKV at 1/1000 dilution in phosphate buffer saline (PBS) solution at 0.135 M and coated overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, High Throughput Screening Assay, Activity Assay